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KMID : 1098420110190020077
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Korean Journal of Medicinal Crop Science
2011 Volume.19 No. 2 p.77 ~ p.82
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Free Radical Scavenging Activity and Protective Effect against H2O2-Induced Stress in Neuronal Cells of Enzymatic Extracts from Sarcodon aspratus
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Lee Seung Jae
Kim Eun-kyung
Oh Hyun-jung
Kwon Hyuck-ju
Hwang Jin-Woo
Moon Sang-Ho
Jeon Byong-Tae
Park Pyo-Jam
Lim Beong-Ou
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Abstract
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The antioxidative activity of various enzymatic extracts from Sarcodon aspratus (S. aspratus) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. For this study, the S. aspratus were enzymatically hydrolyzed by seven carbohydrases (Viscozyme, Celluclast, Dextrozyme, AMG, Promozyme, Maltogenase, and Termamyl) and eight proteases (-chymotrypsin, Alcalase, Flavourzyme, Neutrase, papain, pepsin, Protamax, and trypsin). The DPPH radical scavenging activities of Viscozyme and pepsin extracts were the highest, and the half maximal inhibitory concentration () values were 0.896 and 0.734mg/mL, respectively. The Celluclast and trypsin extracts showed the highest scavenging activities on alkyl radical, and their values were 0.278 and 0.575mg/mL, respectively. The Celluclast extracts was decreased cell apoptosis in PC-12 cells against -induced oxidative damage. The findings of the present study suggest that enzymatic extracts of S. aspratus exhibit antioxidative activity against oxidative stress on PC-12 cells.
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KEYWORD
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Antioxidative Activity, Enzymatic Hydrolysates, Free Radical, S. aspratus, Neuroprotective Effect
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